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<http://fhircat.org/cord-19/metadata/801e028d1c0aa75910dbe03019538ed0dc28b905> fhir_link: <https://fhircat.org/cord-19/fhir/Non-comercial/801e028d1c0aa75910dbe03019538ed0dc28b905> ;
    dc:abstract "In this study, specific sequences within three genes (3D, VP4 and 2B) of the foot-and-mouth disease virus (FMDV) genome were determined to be effective RNAi targets. These sequences are highly conserved among different serotype viruses based on sequence analysis. Small interfering RNA (siRNA)-expressing plasmids (p3D-NT19, p3D-NT56, pVP4-NT19, pVP4-NT65 and p2B-NT25) were constructed to express siRNA targeting 3D, VP4 and 2B, respectively. The antiviral potential of these siRNA for various FMDV isolates was investigated in baby hamster kidney (BHK-21) cells and suckling mice. The results show that these siRNA inhibited virus yield 10- to 300-fold for different FMDV isolates of serotype O and serotype Asia I at 48 h post infection in BHK-21 cells compared to control cells. In suckling mice, p3D-NT56 and p2B-NT25 delayed the death of mice. Twenty percent to 40% of the animals that received a single siRNA dose survived 5 days post infection with serotype O or serotype Asia I. We used an attenuated Salmonella choleraesuis (C500) vaccine strain, to carry the plasmid that expresses siRNA directed against the polymerase gene 3D (p3D-NT56) of FMDV. We used guinea pigs to evaluate the inhibitory effects of recombinant S. cho (p3D-NT56/S. cho) on FMDV infection. The results show that 80% of guinea pigs inoculated with 10(9) CFU of p3D-NT56/S. cho and challenged 36 h later with 50 ID(50) of homologous FMDV were protected. We also measured the antiviral activity of p3D-NT56/S. cho in swine. The results indicate that 100% of the animals treated with 5 × 10(9) CFU of p3D-NT56/S. cho were protected in 9 days." ;
    dc:creator "['Cong, Wei', 'Jin, Hong', 'Jiang, Chengda', 'Yan, Weiyao', 'Liu, Mingqiu', 'Chen, Jiulian', 'Zuo, Xiaoping', 'Zheng, Zhaoxin']" ;
    dc:identifier <http://dx.doi.org/10.1051/vetres/2010002>,
        <https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2826090>,
        <https://www.ncbi.nlm.nih.gov/pubmed/20167192> ;
    dc:issued "2010-01-01"^^xsd:date ;
    dc:license "CC BY-NC" ;
    dc:title "Attenuated Salmonella choleraesuis-mediated RNAi targeted to conserved regions against foot-and-mouth disease virus in guinea pigs and swine" ;
    sso:has_full_text "True" ;
    sso:journal "Vet Res" ;
    sso:sha "801e028d1c0aa75910dbe03019538ed0dc28b905" ;
    sso:source_x "PMC" .