{
   "sha": "41199e837e1c706b7a8a1a8a0970856974235566",
   "source_x": "PMC",
   "title": "Protective Effect and Mechanism of Alprostadil in Acute Respiratory Distress Syndrome Induced by Oleic Acid in Rats",
   "doi": "http://dx.doi.org/10.12659/MSM.909678",
   "pmcid": "PMC6190919",
   "pubmed_id": "30296789",
   "license": "CC BY-NC-ND",
   "abstract": "BACKGROUND: This study investigated the role and mechanism of alprostadil in acute respiratory distress syndrome (ARDS) induced by oleic acid (OA) in rats. MATERIAL/METHODS: Sprague-Dawley rats were randomly divided into control, OA model, and OA + Alprostadil (2.5, 5, and 10 \u03bcg/kg, respectively) groups. The ARDS model was induced by femoral vein injection of OA, and alprostadil was administrated immediately. Lung injury was evaluated by lung wet-dry weight ratio (W/D) and histological analyses. Expressions of ACE, inflammatory mediators, apoptotic-related proteins, and proteins in the MAPKs and NF-\u03baB signaling pathways were determined by Western blot or immunohistochemical staining. RESULTS: Compared with the control group, the OA model group had significantly increased W/D, lung injury score, and collagen deposition at 3 h after OA injection. However, alprostadil (10 \u03bcg/kg) treatment significantly reduced OA-induced elevation of these indicators. Additionally, OA-induced expression of TNF-\u03b1 and IL-1\u03b2 were suppressed by alprostadil. The OA-induced activation of nuclear factor (NF) \u03baB p65 was also reduced by alprostadil. Furthermore, we found that Alprostadil had an inhibitory effect on the phosphorylation of JNK, ERK1/2, and p38 MAPKs. Alprostadil inhibited Bax but increased Bcl-2, indicating a suppressive role in apoptosis. Remarkably increased expression of ACE in the OA model group was observed, which was decreased by alprostadil. CONCLUSIONS: Alprostadil has a protective effect on ARDS induced by OA in rats, possibly through inhibiting apoptosis, suppressing the activation of MAPKs and NF-\u03baB signaling pathways, and decreasing ACE protein expression. Therefore, the use of alprostadil in clinical ARDS treatment is promising.",
   "publish_time": "2018 Oct 8",
   "authors": [
      "['Yan, Xiujuan', 'Li, Yingxiu', 'Choi, Yun Ho', 'Wang, Chongyang', 'Piao, Yihua', 'Ye, Jing', 'Jiang, Jingzhi', 'Li, Liangchang', 'Xu, Huixian', 'Cui, Qingsong', 'Yan, Guanghai', 'Jin, Minggen']"
   ],
   "journal": "Med Sci Monit",
   "has_full_text": "True",
   "id": "41199e837e1c706b7a8a1a8a0970856974235566",
   "fhir_link": "Non-comercial/41199e837e1c706b7a8a1a8a0970856974235566"
}