{
   "sha": "777328a5bc26d551df06e62b64682c03c25dde03",
   "source_x": "PMC",
   "title": "Dendritic Cell-Specific Antigen Delivery by Coronavirus Vaccine Vectors Induces Long-Lasting Protective Antiviral and Antitumor Immunity",
   "doi": "http://dx.doi.org/10.1128/mBio.00171-10",
   "pmcid": "PMC2939679",
   "pubmed_id": "20844609",
   "license": "NO-CC CODE",
   "abstract": "Efficient vaccination against infectious agents and tumors depends on specific antigen targeting to dendritic cells (DCs). We report here that biosafe coronavirus-based vaccine vectors facilitate delivery of multiple antigens and immunostimulatory cytokines to professional antigen-presenting cells in vitro and in vivo. Vaccine vectors based on heavily attenuated murine coronavirus genomes were generated to express epitopes from the lymphocytic choriomeningitis virus glycoprotein, or human Melan-A, in combination with the immunostimulatory cytokine granulocyte-macrophage colony-stimulating factor (GM-CSF). These vectors selectively targeted DCs in vitro and in vivo resulting in vector-mediated antigen expression and efficient maturation of DCs. Single application of only low vector doses elicited strong and long-lasting cytotoxic T-cell responses, providing protective antiviral and antitumor immunity. Furthermore, human DCs transduced with Melan-A-recombinant human coronavirus 229E efficiently activated tumor-specific CD8(+) T cells. Taken together, this novel vaccine platform is well suited to deliver antigens and immunostimulatory cytokines to DCs and to initiate and maintain protective immunity.",
   "publish_time": "2010 Sep 14",
   "authors": [
      "['Cervantes-Barragan, Luisa', 'Z\u00fcst, Roland', 'Maier, Reinhard', 'Sierro, Sophie', 'Janda, Jozef', 'Levy, Frederic', 'Speiser, Daniel', 'Romero, Pedro', 'Rohrlich, Pierre-Simon', 'Ludewig, Burkhard', 'Thiel, Volker']"
   ],
   "journal": "mBio",
   "has_full_text": "True",
   "id": "777328a5bc26d551df06e62b64682c03c25dde03",
   "fhir_link": "PMC/777328a5bc26d551df06e62b64682c03c25dde03"
}