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dc:abstract "DC-SIGN, a C-type lectin expressed on the plasma membrane by human immature dendritic cells, is a receptor for numerous viruses including Ebola, SARS, and dengue. A controversial question has been whether DC-SIGN functions as a complete receptor for both binding and internalization of dengue virus (DENV) or whether it is solely a cell surface attachment factor, requiring either hand-off to another receptor or a co-receptor for internalization. To examine this question, we used four cell types: human immature dendritic cells and NIH3T3 cells expressing either wild type DC-SIGN or two internalization-deficient DC-SIGN mutants, in which either the three cytoplasmic internalization motifs are silenced by alanine substitutions or the cytoplasmic region is truncated. Using confocal and super-resolution imaging and high content single particle tracking, we investigated DENV binding, DC-SIGN surface transport, endocytosis, as well as cell infectivity. DC-SIGN was found colocalized with DENV inside cells suggesting hand-off at the plasma membrane to another receptor did not occur. Moreover, all three DC-SIGN molecules on NIH3T3 cells supported cell infection. These results imply the involvement of a co-receptor because cells expressing the internalization-deficient mutants could still be infected." ;
dc:creator "['Liu, Ping', 'Ridilla, Marc', 'Patel, Pratik', 'Betts, Laurie', 'Gallichotte, Emily', 'Shahidi, Lidea', 'Thompson, Nancy L.', 'Jacobson, Ken']" ;
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dc:title "Beyond attachment: roles of DC-SIGN in dengue virus infection" ;
sso:source_x "PMC" .