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<https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4390637> dc:abstract "A method for the rapid diagnosis of early dengue virus (DENV) infection is highly needed. Here, a prototype reverse transcription-recombinase polymerase amplification (RT-RPA) assay was developed. The assay detected DENV RNA in <20 min without the need for thermocycling amplification. The assay enabled the detection of as few as 10 copies of DENV RNA. The designed RT-RPA primers and exo probe detected the DENV genome of at least 12 genotypes of DENV circulating globally without cross-reacting with other arboviruses. We assessed the diagnostic performance of the RT-RPA assay for the detection of DENV RNA in 203 serum samples of patients with clinically suspected dengue. The sera were simultaneously tested for DENV using a reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay, quantitative RT-PCR (qRT-PCR), and IgM- and IgG-capture enzyme-linked immunosorbent assays (ELISA). Acute DENV infection was confirmed in 130 samples and 61 of the samples (46.9%) were classified as viremic with qRT-PCR. The RT-RPA assay showed good concordance (κ of ≥0.723) with the RT-LAMP and qRT-PCR assays in detecting the dengue viremic samples. When used in combination with ELISA, both the RT-RPA and RT-LAMP assays increased the detection of acute DENV infection to ≥95.7% (≥45/47) in samples obtained within 5 days of illness. The results from the study suggest that the RT-RPA assay is the most rapid molecular diagnostic tool available for the detection of DENV. Hence, it is possible to use the RT-RPA assay in a laboratory to complement routine serology testing for dengue." ;
    dc:creator "['Teoh, Boon-Teong', 'Sam, Sing-Sin', 'Tan, Kim-Kee', 'Danlami, Mohammed Bashar', 'Shu, Meng-Hooi', 'Johari, Jefree', 'Hooi, Poh-Sim', 'Brooks, David', 'Piepenburg, Olaf', 'Nentwich, Oliver', 'Wilder-Smith, Annelies', 'Franco, Leticia', 'Tenorio, Antonio', 'AbuBakar, Sazaly']" ;
    dc:identifier <http://dx.doi.org/10.1128/JCM.02648-14>,
        <https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4390637> ;
    dc:title "Early Detection of Dengue Virus by Use of Reverse Transcription-Recombinase Polymerase Amplification" ;
    sso:source_x "PMC" .