{
   "source_x": "PMC",
   "title": "New Pre-pandemic Influenza Vaccines: An Egg-and Adjuvant-independent Human Adenoviral Vector Strategy Induces Long-lasting Protective Immune Responses in Mice",
   "doi": "http://dx.doi.org/10.1038/sj.clpt.6100418",
   "pmcid": "PMC2793094",
   "abstract": "Highly pathogenic avian H5N1 influenza viruses that are currently circulating in southeast Asia may acquire the potential to cause the next influenza pandemic. A number of alternate approaches are being pursued to generate cross-protective, dose-sparing, safe, and effective vaccines, as traditional vaccine approaches, i.e., embryonated egg-grown, are not immunogenic. We developed a replication-incompetent adenoviral vector-based, adjuvant-and egg-independent pandemic influenza vaccine strategy as a potential alternative to conventional egg-derived vaccines. In this paper, we address suboptimal dose and longevity of vaccine-induced protective immunity and demonstrate that a vaccine dose as little as 1 \u00d7 10(6) plaque-forming unit (PFU) is sufficient to induce protective immune responses against a highly pathogenic H5N1 virus. Furthermore, the vaccine-induced humoral and cellular immune responses and protective immunity persisted at least for a year.",
   "authors": [
      "['Hoelscher, MA', 'Jayashankar, L', 'Garg, S', 'Veguilla, V', 'XLu', 'Singh, N', 'Katz, JM', 'Mittal, SK', 'Sambhara, S']"
   ],
   "id": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2793094"
}