{
   "source_x": "PMC",
   "title": "Efficient Replication of Severe Acute Respiratory Syndrome Coronavirus in Mouse Cells Is Limited by Murine Angiotensin-Converting Enzyme 2",
   "doi": "http://dx.doi.org/10.1128/JVI.78.20.11429-11433.2004",
   "pmcid": "PMC521845",
   "abstract": "Replication of viruses in species other than their natural hosts is frequently limited by entry and postentry barriers. The coronavirus that causes severe acute respiratory syndrome (SARS-CoV) utilizes the receptor angiotensin-converting enzyme 2 (ACE2) to infect cells. Here we compare human, mouse, and rat ACE2 molecules for their ability to serve as receptors for SARS-CoV. We found that, compared to human ACE2, murine ACE2 less efficiently bound the S1 domain of SARS-CoV and supported less-efficient S protein-mediated infection. Rat ACE2 was even less efficient, at near background levels for both activities. Murine 3T3 cells expressing human ACE2 supported SARS-CoV replication, whereas replication was less than 10% as efficient in the same cells expressing murine ACE2. These data imply that a mouse transgenically expressing human ACE2 may be a useful animal model of SARS.",
   "authors": [
      "['Li, Wenhui', 'Greenough, Thomas C.', 'Moore, Michael J.', 'Vasilieva, Natalya', 'Somasundaran, Mohan', 'Sullivan, John L.', 'Farzan, Michael', 'Choe, Hyeryun']"
   ],
   "id": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC521845"
}