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<http://fhircat.org/cord-19/metadata/267729947ca478946d5a4bffb8e13d50c3545120> fhir_link: <https://fhircat.org/cord-19/fhir/bioRxiv-medRxiv/267729947ca478946d5a4bffb8e13d50c3545120> ;
    dc:abstract "<jats:p>Corona Virus Disease 2019 (COVID-19) is a recently emerged life-threatening disease caused by SARS-CoV-2. Real- time fluorescent PCR (RT-PCR) is the clinical standard for SARS-CoV-2 nucleic acid detection. To detect SARS-CoV-2 early and control the disease spreading on time, a faster and more convenient method for SARS-CoV-2 nucleic acid detecting, RT-LAMP method (reverse transcription loop-mediated isothermal amplification) was developed. RNA reverse transcription and nucleic acid amplification were performed in one step at 63 ℃ isothermal conditions, and the results can be obtained within 30 minutes. ORF1ab gene, E gene and N gene were detected at the same time. ORF1ab gene was very specific and N gene was very sensitivity, so they can guarantee both sensitivity and specificity for SARS-CoV-2. The sensitivity of RT-LAMP assay is similar to RT-PCR, and specificity was 99% as detecting 208 clinical specimens. The RT-LAMP assay reported here has the advantages of rapid amplification, simple operation, and easy detection, which is useful for the rapid and reliable clinical diagnosis of SARS-CoV-2.</jats:p>" ;
    dc:creator "['Weihua Yang', 'Xiaofei Dang', 'Qingxi Wang', 'Mingjie Xu', 'Qianqian Zhao', 'Yunying Zhou', 'Huailong Zhao', 'Li Wang', 'Yihui Xu', 'Jun Wang', 'Shuyi Han', 'Min Wang', 'Fengyan Pei', 'Yunshan Wang']" ;
    dc:identifier <https://doi.org/doi.org/10.1101/2020.03.02.20030130> ;
    dc:issued "2020-03-02"^^xsd:date ;
    dc:license "See https://www.medrxiv.org/submit-a-manuscript" ;
    dc:title "Rapid Detection of SARS-CoV-2 Using Reverse transcription RT-LAMP method" ;
    sso:has_full_text "True" ;
    sso:sha "267729947ca478946d5a4bffb8e13d50c3545120" ;
    sso:source_x "medrxiv" .