{
   "sha": "c0f05769898b94c3e481a16c7fee9666037bbdab",
   "source_x": "PMC",
   "title": "Isolation and characterization of a variant porcine epidemic diarrhea virus in China",
   "doi": "http://dx.doi.org/10.1186/1743-422X-9-195",
   "pmcid": "PMC3487931",
   "pubmed_id": "22967434",
   "license": "CC BY",
   "abstract": "An outbreak of diarrhea in pigs started in Guangdong, South China in January 2011. Cases were characterized by watery diarrhea, dehydration and vomiting, with 80\u2013100% morbidity and 50\u201390% mortality in suckling piglets. The causative agent of the diarrhea was ultimately identified as porcine epidemic diarrhea virus (PEDV). In this study, we isolated a PEDV strain designated CHGD-01 from piglet intestines using Vero cell cultures, and its specific cytopathic effects were confirmed in susceptible cells by direct immunofluorescence testing and electron microscopy. The complete genome of CHGD-01 was shown to be 28,035 nucleotides in length, with a similar structure to that of PEDV reference strains. Phylogenetic analyses based on the whole genome revealed that CHGD-01 shared nucleotide sequence identities of 98.2\u201398.4% with two other Chinese isolates reported in the same year, thus constituting a new cluster. Amino acid sequence analysis based on individual virus genes indicated a close relationship between the spike protein gene of CHGD-01 and the field strain KNU0802 in Korea. Its ORF3 and nucleoprotein genes, however, were divergent from all other sequenced PEDV isolate clusters and therefore formed a new group, suggesting a new variant PEDV isolate in China. Further studies will be required to determine the immunogenicity and pathogenicity of this new variant.",
   "publish_time": "2012 Sep 12",
   "authors": [
      "['Pan, Yongfei', 'Tian, Xiaoyan', 'Li, Wei', 'Zhou, Qingfeng', 'Wang, Dongdong', 'Bi, Yingzuo', 'Chen, Feng', 'Song, Yanhua']"
   ],
   "journal": "Virol J",
   "has_full_text": "True",
   "id": "c0f05769898b94c3e481a16c7fee9666037bbdab",
   "fhir_link": "Commercial/c0f05769898b94c3e481a16c7fee9666037bbdab"
}