{
   "sha": "d2d7428869cef29308728dd3a05f0facaf1d25da",
   "source_x": "PMC",
   "title": "SARS-CoV envelope protein palmitoylation or nucleocapid association is not required for promoting virus-like particle production",
   "doi": "http://dx.doi.org/10.1186/1423-0127-21-34",
   "pmcid": "PMC4014084",
   "pubmed_id": "24766657",
   "license": "CC BY",
   "abstract": "BACKGROUND: Coronavirus membrane (M) proteins are capable of interacting with nucleocapsid (N) and envelope (E) proteins. Severe acute respiratory syndrome coronavirus (SARS-CoV) M co-expression with either N or E is sufficient for producing virus-like particles (VLPs), although at a lower level compared to M, N and E co-expression. Whether E can release from cells or E/N interaction exists so as to contribute to enhanced VLP production is unknown. It also remains to be determined whether E palmitoylation or disulfide bond formation plays a role in SARS-CoV virus assembly. RESULTS: SARS-CoV N is released from cells through an association with E protein-containing vesicles. Further analysis suggests that domains involved in E/N interaction are largely located in both carboxyl-terminal regions. Changing all three E cysteine residues to alanines did not exert negative effects on E release, E association with N, or E enhancement of VLP production, suggesting that E palmitoylation modification or disulfide bond formation is not required for SARS-CoV virus assembly. We found that removal of the last E carboxyl-terminal residue markedly affected E release, N association, and VLP incorporation, but did not significantly compromise the contribution of E to efficient VLP production. CONCLUSIONS: The independence of the SARS-CoV E enhancement effect on VLP production from its viral packaging capacity suggests a distinct SARS-CoV E role in virus assembly.",
   "publish_time": "2014 Apr 27",
   "authors": [
      "['Tseng, Ying-Tzu', 'Wang, Shiu-Mei', 'Huang, Kuo-Jung', 'Wang, Chin-Tien']"
   ],
   "journal": "J Biomed Sci",
   "has_full_text": "True",
   "id": "d2d7428869cef29308728dd3a05f0facaf1d25da",
   "fhir_link": "Commercial/d2d7428869cef29308728dd3a05f0facaf1d25da"
}