{
   "sha": "d5d7c5ada2462aab2fa09789b1ef5ffe8e7f8d64",
   "source_x": "PMC",
   "title": "Diagnosis of cytomegalovirus pneumonia by quantitative polymerase chain reaction using bronchial washing fluid from patients with hematologic malignancies",
   "doi": "http://dx.doi.org/10.18632/oncotarget.14504",
   "pmcid": "PMC5503648",
   "pubmed_id": "28061469",
   "license": "CC BY",
   "abstract": "BACKGROUND: The incidence of cytomegalovirus (CMV) pneumonia is increasing in patients diagnosed with hematologic malignancies. The utility of CMV-DNA viral load measurement has not been standardized, and viral cut-off values have not been established. This study was designed to investigate the utility of CMV quantitative real-time PCR (qRT-PCR) using bronchial washing fluid. METHODS: We retrospectively reviewed the microbiologic and pathologic results of bronchial washing fluid and biopsy specimens in addition to the patients' clinical characteristics. RESULTS: A total of 565 CMV qRT-PCR assays were performed using bronchial washing fluid from patients with hematologic malignancies. Among them, 101 were positive for CMV by qRT-PCR; of these, 24 were diagnosed with CMV pneumonia and 70 with CMV infection, and 7 were excluded due to a diagnosis of invasive pulmonary aspergillosis rather than viral pneumonia. The median CMV load determined by qPCR was 1.8 \u00d7 10(5) copies/mL (3.6 10(3)-1.5 \u00d7 10(8)) in CMV pneumonia patients and 3.0 \u00d7 10(3) copies/mL (5.0 \u00d7 10(2)-1.1 \u00d7 10(5)) in those diagnosed with CMV infection (P < 0.01). Using the ROC curve, the optimal inflection points were 18,900 copies/mL (137,970 IU/mL) in post-bone marrow transplantation (BMT) patients, 316,415 copies/mL (2,309,825 IU/mL) in no-BMT patients and 28,774 copies/mL (210,054 IU/mL) in all patients. CONCLUSIONS: The CMV titers in bronchial washing fluid determined by qRT-PCR differed significantly between patients diagnosed with CMV pneumonia and those with CMV infection. The viral cut-off values in bronchial washing fluid were suggested for the diagnosis of CMV pneumonia, which were different depending on the BMT status.",
   "publish_time": "2017 Jan 4",
   "authors": [
      "['Lee, Hwa Young', 'Rhee, Chin Kook', 'Choi, Joon Young', 'Lee, Hea Yon', 'Lee, Jong Wook', 'Lee, Dong Gun']"
   ],
   "journal": "Oncotarget",
   "has_full_text": "True",
   "id": "d5d7c5ada2462aab2fa09789b1ef5ffe8e7f8d64",
   "fhir_link": "Commercial/d5d7c5ada2462aab2fa09789b1ef5ffe8e7f8d64"
}