{
   "sha": "e5ec05c6d25d098703bee2ff49b0e02d0bfa9de5",
   "source_x": "PMC",
   "title": "Myeloablation-associated deletion of ORF4 in a human coronavirus 229E infection",
   "doi": "http://dx.doi.org/10.1038/s41525-017-0033-4",
   "pmcid": "PMC5677986",
   "pubmed_id": "29263840",
   "license": "CC BY",
   "abstract": "We describe metagenomic next-generation sequencing (mNGS) of a human coronavirus 229E from a patient with AML and persistent upper respiratory symptoms, who underwent hematopoietic cell transplantation (HCT). mNGS revealed a 548-nucleotide deletion, which comprised the near entirety of the ORF4 gene, and no minor allele variants were detected to suggest a mixed infection. As part of her pre-HCT conditioning regimen, the patient received myeloablative treatment with cyclophosphamide and 12\u2009Gy total body irradiation. Iterative sequencing and RT-PCR confirmation of four respiratory samples over the 4-week peritransplant period revealed that the pre-conditioning strain contained an intact ORF4 gene, while the deletion strain appeared just after conditioning and persisted over a 2.5-week period. This sequence represents one of the largest genomic deletions detected in a human RNA virus and describes large-scale viral mutation associated with myeloablation for HCT.",
   "publish_time": "2017 Oct 9",
   "authors": [
      "['Greninger, Alexander L.', 'Pepper, Gregory', 'Shean, Ryan C.', 'Cent, Anne', 'Palileo, Isabel', 'Kuypers, Jane M.', 'Schiffer, Joshua T.', 'Jerome, Keith R.']"
   ],
   "journal": "NPJ Genom Med",
   "has_full_text": "False",
   "id": "e5ec05c6d25d098703bee2ff49b0e02d0bfa9de5"
}