{
   "sha": "6387603fb5f8eda220411438a86234c475aa728e",
   "source_x": "PMC",
   "title": "M(IL-4) Tissue Macrophages Support Efficient Interferon-Gamma Production in Antigen-Specific CD8(+) T Cells with Reduced Proliferative Capacity",
   "doi": "http://dx.doi.org/10.3389/fimmu.2017.01629",
   "pmcid": "PMC5714867",
   "pubmed_id": "29250063",
   "license": "CC BY",
   "abstract": "CD8(+) cytotoxic T cell (CTL) responses are necessary for the lysis of virally infected cells and control of infection. CTLs are activated when their TCRs bind a major histocompatibility complex (MHC)-I/peptide complex on the surface of antigen presenting cells such as macrophages (M\u03a6). It is now apparent that M\u03a6 display remarkable plasticity in response to environmental signals to polarize into classically activated M(LPS\u2009+\u2009IFN-\u03b3) or alternatively activated M(IL-4). However, little is known about how M\u03a6 activation status influences their antigen presentation function to CD8(+) T cell in models of virus infection. Consequently, we tested how polarization of spleen-derived (Sp)-M\u03a6 impacts direct presentation of viral antigens to influence effector and proliferative CD8(+) T-cell responses. We show that M(IL-4) Sp-M\u03a6 retain MHC-I surface expression and the ability to stimulate IFN-\u03b3 production by CTL following peptide stimulation and lymphocytic choriomeningitis virus infection to levels similar to M0 and M(LPS\u2009+\u2009IFN-\u03b3) M\u03a6. However, memory CD8(+) T cells cultured in the presence of M(IL-4) M\u03a6 underwent significantly reduced proliferation and produced similar IFN-\u03b3 levels as coculturing with M0 or M(LPS\u2009+\u2009IFN-\u03b3) cells. Thus, these results show a novel ability of polarized M\u03a6 to regulate CD8(+) T-cell proliferation and effector functions during virus infection.",
   "publish_time": "2017 Nov 30",
   "authors": [
      "['Mulder, Rylend', 'Banete, Andra', 'Seaver, Kyle', 'Basta, Sameh']"
   ],
   "journal": "Front Immunol",
   "has_full_text": "True",
   "id": "6387603fb5f8eda220411438a86234c475aa728e",
   "fhir_link": "Commercial/6387603fb5f8eda220411438a86234c475aa728e"
}