{
   "sha": "32d5b5c7b38c1dd14b316de97bce86e5ab252f91",
   "source_x": "PMC",
   "title": "Development and characterization of serotype-specific monoclonal antibodies against the dengue virus-4 (DENV-4) non-structural protein (NS1)",
   "doi": "http://dx.doi.org/10.1186/s12985-018-0925-7",
   "pmcid": "PMC5801815",
   "pubmed_id": "29409531",
   "license": "CC BY",
   "abstract": "BACKGROUND: Dengue, caused by one of the four serologically distinct dengue viruses (DENV-1 to \u2212\u20094), is a mosquito-borne disease of serious global health significance. Reliable and cost-effective diagnostic tests, along with effective vaccines and vector-control strategies, are highly required to reduce dengue morbidity and mortality. Evaluation studies revealed that many commercially available NS1 antigen (Ag) tests have limited sensitivity to DENV-4 serotype compared to the other three serotypes. These studies indicated the need for development of new NS1 Ag detection test with improved sensitivity to DENV-4. An NS1 capture enzyme linked immunoassay (ELISA) specific to DENV-4 may improve the detection of DENV-4 cases worldwide. In addition, a serotype-specific NS1 Ag test identifies both DENV and the infecting serotype. METHODS: In this study, we used a small-ubiquitin-like modifier (SUMO*) cloning vector to express a SUMO*-DENV-4 rNS1 fusion protein to develop NS1 DENV-4 specific monoclonal antibodies (MAbs). These newly developed MAbs were then optimized for use in an anti-NS1 DENV-4 capture ELISA. The serotype specificity and sensitivity of this ELISA was evaluated using (i) supernatants from DENV (1\u20134)-infected Vero cell cultures, (ii) rNS1s from all the four DENV (1\u20134) and, (iii) rNS1s of related flaviviruses (yellow fever virus; YFV and West Nile virus; WNV). RESULTS: From the evaluation studies of the newly developed MAbs, we identified three DENV-4 specific anti-NS1 MAbs: 3H7A9, 8A6F2 and 6D4B10. Two of these MAbs were optimal for use in a DENV-4 serotype-specific NS1 capture ELISA: MAb 8A6F2 as the capture antibody and 6D4B10 as a detection antibody. CONCLUSION: This ELISA was sensitive and specific to DENV-4 with no cross-reactivity to other three DENV (1\u20133) serotypes and other heterologous flaviviruses. Taken together these data indicated that our MAbs are useful reagents for the development of DENV-4 immunodiagnostic tests. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi: 10.1186/s12985-018-0925-7) contains supplementary material, which is available to authorized users.",
   "publish_time": "2018 Feb 6",
   "authors": [
      "['Gelanew, Tesfaye', 'Hunsperger, Elizabeth']"
   ],
   "journal": "Virol J",
   "has_full_text": "True",
   "id": "32d5b5c7b38c1dd14b316de97bce86e5ab252f91",
   "fhir_link": "Commercial/32d5b5c7b38c1dd14b316de97bce86e5ab252f91"
}