{
   "sha": "9f88a88a26c8432f7c3dea1a2f8d1b330a559d48",
   "source_x": "PMC",
   "title": "Development of a cell-based assay to identify hepatitis B virus entry inhibitors targeting the sodium taurocholate cotransporting polypeptide",
   "doi": "http://dx.doi.org/10.18632/oncotarget.25348",
   "pmcid": "PMC5955094",
   "pubmed_id": "29805766",
   "license": "CC BY",
   "abstract": "Sodium taurocholate cotransporting polypeptide (NTCP) is a major entry receptor of hepatitis B virus (HBV) and one of the most attractive targets for anti-HBV drugs. We developed a cell-mediated drug screening method to monitor NTCP expression on the cell surface by generating a HepG2 cell line with tetracycline-inducible expression of NTCP and a monoclonal antibody that specifically detects cell-surface NTCP. Using this system, we screened a small molecule library for compounds that protected against HBV infection by targeting NTCP. We found that glabridin, a licorice-derived isoflavane, could suppress viral infection by inducing caveolar endocytosis of cell-surface NTCP with an IC(50) of ~40 \u03bcM. We also found that glabridin could attenuate the inhibitory effect of taurocholate on type I interferon signaling by depleting the level of cell-surface NTCP. These results demonstrate that our screening system could be a powerful tool for discovering drugs targeting HBV entry.",
   "publish_time": "2018 May 4",
   "authors": [
      "['Miyakawa, Kei', 'Matsunaga, Satoko', 'Yamaoka, Yutaro', 'Dairaku, Mina', 'Fukano, Kento', 'Kimura, Hirokazu', 'Chimuro, Tomoyuki', 'Nishitsuji, Hironori', 'Watashi, Koichi', 'Shimotohno, Kunitada', 'Wakita, Takaji', 'Ryo, Akihide']"
   ],
   "journal": "Oncotarget",
   "has_full_text": "False",
   "id": "9f88a88a26c8432f7c3dea1a2f8d1b330a559d48"
}